Journal: Cell reports

Article Title: CRACD Loss Induces Neuroendocrine Cell Plasticity of Lung Adenocarcinoma

doi: 10.1016/j.celrep.2024.114286

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat anti-CD326 (EPCAM) MicroBeads, mouse , Miltenyi Biotec , Cat# 130-105-958; RRID: AB 2936423.

Techniques: Virus, Plasmid Preparation, Recombinant, Modification, cDNA Synthesis, Staining, Cell Recovery, Infection, Transfection, shRNA, Software

Journal: Oncotarget

Article Title: Renal cell carcinoma alters endothelial receptor expression responsible for leukocyte adhesion

doi: 10.18632/oncotarget.7804

Figure Lengend Snippet: A. Magnet bead separation of Caki-1 and HUVEC from the co-culture by epithelial CD326. Black line: isotype control, green line: unstimulated cells, red line: TNF-alpha (24h, 500 U/ml) stimulated cells. One representative of three separate experiments is shown. B. Western Blot Analysis. Total protein content of ICAM-1, VCAM-1, E-selectin and CD144 (endothelial control) in Caki-1 and HUVEC cells separated by magnet beads. HUVEC + Caki = HUVEC separated from Caki-1 co-culture. Caki-1 and HUVEC cells, cultivated separately, served as controls. n.d. = not detectable. C. Pixel density of protein bands shown in B.

Article Snippet: For Western blot analysis Caki-1 were separated from co-culture with HUVEC by magnet bead separation with human CD326 MicroBeads (EpCam = epithelial cell adhesion molecule, 100 μl, Miltenyi Biotec, Bergisch Gladbach, Germany) using a MidiMACS Separator (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Co-Culture Assay, Control, Western Blot

Journal: Frontiers in oncology

Article Title: Membrane Hsp70-A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition.

doi: 10.3389/fonc.2018.00497

Figure Lengend Snippet: FIGURE 1 | Membrane Hsp70 and EpCAM expression by different cancer cell lines. Cell lines from different cancer entities (breast, lung, melanoma, pancreas, cervix, colon, brain, head, and neck) were screened for their expression of mHsp70 and EpCAM using flow cytometry. Gray histograms represent isotype control, white histograms mHsp70 staining and black histograms EpCAM staining. The numbers in the histograms indicate the proportion of cells stained positively for mHsp70 and EpCAM. The data show one representative result of at least three independent experiments.

Article Snippet: At the Technische Universität München, flow cytometric analysis for the expression of mHsp70 and EpCAM on tumor cell lines was undertaken using either an FITC-conjugated cmHsp70.1 mAb (multimmune GmbH, Munich, Germany) and a FITCconjugated EpCAM (CD326, clone HEA125) and PE-conjugated EpCAM (CD326, clone G8.8) mAb (Acris GmbH, an OriGene Company, Herford, Germany).

Techniques: Membrane, Expressing, Cytometry, Control, Staining

Journal: Frontiers in oncology

Article Title: Membrane Hsp70-A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition.

doi: 10.3389/fonc.2018.00497

Figure Lengend Snippet: FIGURE 2 | Influence of TGFβ- and L-lactic-acid (Lac-Ac)-induced EMT on mHsp70 and EpCAM expression by DU145 (prostate), UP154 (head and neck), UD5 (head and neck), A549 (lung) cancer cells, and LS174T melanoma cells. (A) DU145 cells were cultured with TGFβ for 10 days, UP154 and UD5 cells were cultured for 2 days with L-lactic-acid (Lac-Ac), after which the expression of mHsp70 and EpCAM was determined. (B) A549 and LS174T cells were cultured either with TGFβ for 10 days or with L-lactic-acid (Lac-Ac) for 2 days, after which the expression of mHsp70 and EpCAM was determined on viable cells using flow cytometry. Moreover, non-adherent and adherent cells were analyzed separately in A549 cells for their mHsp70 and EpCAM expression. Gray histograms represent isotype control, white histograms mHsp70 staining and black histograms EpCAM staining. The proportion of cells stained positively for mHsp70 and EpCAM are indicated in each histogram. The data show one representative result of at least three independent experiments. (C) Representative photomicrographs of untreated and TGFβ treated A549 cells on day 10, day 10 + 4 days recovery without TGFβ and day 10 + 7 days recovery without TGFβ.

Article Snippet: At the Technische Universität München, flow cytometric analysis for the expression of mHsp70 and EpCAM on tumor cell lines was undertaken using either an FITC-conjugated cmHsp70.1 mAb (multimmune GmbH, Munich, Germany) and a FITCconjugated EpCAM (CD326, clone HEA125) and PE-conjugated EpCAM (CD326, clone G8.8) mAb (Acris GmbH, an OriGene Company, Herford, Germany).

Techniques: Expressing, Cell Culture, Cytometry, Control, Staining

Journal: Frontiers in oncology

Article Title: Membrane Hsp70-A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition.

doi: 10.3389/fonc.2018.00497

Figure Lengend Snippet: FIGURE 5 | Capture of SK-BR-3 tumor cells with the cmHsp70.1 mAb-functionalized CellCollector® system and subsequent cultivation and analysis. SK-BR-3 cells were captured from a mixture of the tumor cell line with EDTA blood. The functional part of the wire was cut from the detector and transferred to a cell culture flask. Cells that became adherent in the flask were expanded and finally analyzed with flow cytometry. Cells captured with the cmHsp70.1 mAb-functionalized wire (right panel) showed characteristics comparable to those of SK-BR-3 cells from cell culture (left panel). Gray histograms represent isotype control, white histograms mHsp70 staining and black histograms EpCAM staining. The numbers in the histograms indicate the proportion of cells stained positive for mHsp70 and EpCAM. Data are a representative result of at least three independent experiments.

Article Snippet: At the Technische Universität München, flow cytometric analysis for the expression of mHsp70 and EpCAM on tumor cell lines was undertaken using either an FITC-conjugated cmHsp70.1 mAb (multimmune GmbH, Munich, Germany) and a FITCconjugated EpCAM (CD326, clone HEA125) and PE-conjugated EpCAM (CD326, clone G8.8) mAb (Acris GmbH, an OriGene Company, Herford, Germany).

Techniques: Functional Assay, Cell Culture, Cytometry, Control, Staining

Journal: Frontiers in oncology

Article Title: Membrane Hsp70-A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition.

doi: 10.3389/fonc.2018.00497

Figure Lengend Snippet: FIGURE 6 | Capture of CTCs from the EDTA blood of patients using EpCAM mAb- or cmHsp70.1 mAb-functionalized magnetic beads. Representative views of singular (A) and clustered (B) CTCs derived from EDTA blood (7.5 ml, each) of patients with squamous cell carcinoma of the head and neck (SCCHN) and non-small cell lung carcinoma (NSCLC) bound to the EpCAM mAb- and cmHsp70.1 mAb-functionalized beads: brightfield, DAPI (blue), FITC-labeled cytokeratin antibody (CK-FITC, green), PE-labeled EpCAM (EpCAM-PE, red), overlay derived thereof. Scale bar 20 µm.

Article Snippet: At the Technische Universität München, flow cytometric analysis for the expression of mHsp70 and EpCAM on tumor cell lines was undertaken using either an FITC-conjugated cmHsp70.1 mAb (multimmune GmbH, Munich, Germany) and a FITCconjugated EpCAM (CD326, clone HEA125) and PE-conjugated EpCAM (CD326, clone G8.8) mAb (Acris GmbH, an OriGene Company, Herford, Germany).

Techniques: Magnetic Beads, Derivative Assay, Labeling